ABSTRACT
Preparaçöes antigenicas soluveis de Schistosoma mansoni adulto (SWAP) fixadas covalentemente em discos de polivinil alcool-glutaraldeido foram usadas em teste de ELISA. As melhores condiçöes para o ensaio foram estabelecidas e foi encontrado que uma quantidade mínima de antigeno, em torno de 1,5 ug e necessária. Comparaçäo entre este procedimento e o de ELISA convencional foi estabelecida. Um metodo seguro de imobilizaçäo do antigeno foi alcançado e os baixos preços dos reagentes empregados säo economicamente atraentes...
Subject(s)
Humans , Polyvinyl Alcohol , Schistosoma mansoni/drug effects , Schistosomiasis mansoni/diagnosis , Enzyme-Linked Immunosorbent Assay , Evaluation Study , GlutaralABSTRACT
Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3µg of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3 per cent w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.